We narrowed to 10,500 results for: fus
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Plasmid#195166Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-mNG
Plasmid#195167Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-Halo
Plasmid#195168Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
TagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-Halo
Plasmid#195169Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tagDepositorInsertABHD16A (ABHD16A Human)
TagsHalo tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Mouse-caspase-1/11a
Plasmid#183391PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Mouse, Synthetic)
UseRetroviralTagsMycPromoterMESVAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15541_dCBE-SuperFi-Cas9
Plasmid#184371PurposeMammalian expression of nuclease inactive SuperFi-Cas9 CBE base editorDepositorInsertdCBE-SuperFi-Cas9
UseCRISPRTags3X FLAG, SV40 NLS, and UGIExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15543_dABE-SuperFi-Cas9
Plasmid#184373PurposeMammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editorDepositorInsertdABE-SuperFi-Cas9
UseCRISPRTagsFLAG, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15545_dABE8e-SuperFi-Cas9
Plasmid#184375PurposeMammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editorDepositorInsertdABE8e-SuperFi-Cas9
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationD10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…PromoterEF-1α core promoterAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain
Plasmid#183396PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain
Plasmid#183395PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
UseRetroviralTagsMycPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24-ORF24-2xStrep
Plasmid#138453PurposeExpresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24-ORF24 (ORF24 MHV68/KSHV)
TagsStrepExpressionMammalianMutationmu24 amino acids 1-191 fused to ORF24 amino acids…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1-ORF24-2xStrep
Plasmid#138454PurposeExpresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorTagsStrepExpressionMammalianMutationBcRF1 amino acids 1-178 fused to ORF24 amino acid…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87-ORF24-2xStrep
Plasmid#138455PurposeExpresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cellsDepositorTagsStrepExpressionMammalianMutationUL87 amino acids 1-248 fused to ORF24 amino acids…PromoterCMVAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-mCherr
Plasmid#140307PurposeExpresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationcontains only the SH4 and UD regions fused to mCh…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only