We narrowed to 16,183 results for: nes
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Plasmid#206020PurposeAn intact type LINE-1 vector plasmid possessing the scFv-Fc expression unitDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit
ExpressionMammalianAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)
Plasmid#193854PurposePromoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing padDepositorInsert5' Cbr-unc-119(+) + 5' mNeonGreen
UseCRISPR and Cre/LoxExpressionWormPromoterCbr-unc-119pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-Myr-PTEN
Plasmid#206983PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signalDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsN-terminal myristoylation siteExpressionMammalianPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
ExpressionPlantPromoterCaMV 35SAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-KAP3B
Plasmid#193939PurposeMammalian expression of KAP 3BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKAP 3B (KIFAP3 Human)
TagsFRBExpressionMammalianPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-i5-msfGFP
Plasmid#180332Purposemammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEPTIN9_v7 (SEPTIN9 Human)
TagsmsfGFPExpressionMammalianMutationSEPT9_i1-i5PromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBI
Plasmid#176051PurposeExpresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyc
Tags6xHis and V5ExpressionMammalianMutationdeleted amino acids 44-63PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMB0
Plasmid#176050PurposeExpresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyc
Tags6xHis and V5ExpressionMammalianMutationdeleted amino acids 16-33PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertretroCHMP3
TagsHA and T2A-H2B-BFPExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC1-1-170
Plasmid#173467PurposePGRMC1 lacking the extreme C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPGRMC1 (PGRMC1 Human)
TagsFLAGExpressionMammalianMutationDeleted for amino acids 171-194Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170814PurposeLentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreenDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP803
Plasmid#164547PurposeExpresses Smo-FLAG K434, 444, 448RDepositorInsertSmoothened K434, 444, 448R (Smo Mouse)
UseLentiviralTagsFLAGMutationAll three Lysines in cytoplasmic loop three of Sm…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-CRISPR-TagV2
UseCRISPRExpressionMammalianAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159848PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159856PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only