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3xFLAG-CUL1-pCMV7.1

Plasmid

#155019

Purpose

N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 1 (CUL1 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2a KI

Plasmid

#131486

Purpose

Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-ChR2-YFP

Plasmid

#110337

Purpose

ChR2-YFP reporter vector of iTango and Cal-Light system for AAV production

Depositor

Hyungbae Kwon

Insert

tetO-Flex-ChR2-YFP

Use

AAV

Expression

Mammalian

Mutation

Chr2 H134R

Promoter

tetO

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-mitfa2.1

Plasmid

#81234

Purpose

zebrafish mitfa promoter entry vector for multisite Gateway cloning

Depositor

James Lister

Insert

mitfa promoter (mitfa Zebrafish)

Use

Multisite gateway entry vector

Promoter

mitfa, 2.1kb including 5' UTR

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP-T2A-mCherry

Plasmid

#80347

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCherry

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Renilla:Tnos (GB0109)

Plasmid

#68215

Purpose

Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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Punc-4::NGL-1::spGFP1-10

Plasmid

#65827

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP1-10

Expression

Worm

Promoter

Punc-4

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSS146

Plasmid

#64770

Purpose

For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH4

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chrimson-GFP

Plasmid

#62718

Purpose

AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-N

Plasmid

#53767

Purpose

mammalian expression of flag-tagged ATR N-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal domain, aa 1-1409

Promoter

CMV

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-C7orf59

Plasmid

#42336

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-shSmad4

Plasmid

#37046

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad4 (SMAD4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hFrataxin-HA

Plasmid

#31895

Depositor

Gino Cortopassi

Article

Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

Insert

Frataxin (FXN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pNCS

Plasmid

#17362

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

Insert

MMLV proviral DNA

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-VChR1-EYFP-WPRE

Plasmid

#20954

Purpose

3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-Esg1

Plasmid

#13352

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 5 (Dppa5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
EE-mut SV40 luc

Plasmid

#8460

Depositor

Bob Weinberg

Article

Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

Insert

human cyclin E promoter fragment, mutant (CCNE1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

E2F sites I, II, and III mutated.

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only