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pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

Plasmid

#196617

Purpose

Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BJ.1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7V51G-VA

Plasmid

#115183

Purpose

Lentiviral transduction and expression of PARK7V51G into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7V51G (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.V51G

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7C53A-VA

Plasmid

#115184

Purpose

Lentiviral transduction and expression of PARK7C53A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7C53A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.C53A

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7H126A-VA

Plasmid

#115185

Purpose

Lentiviral transduction and expression of PARK7H126A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.H126A

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7E163K-VA

Plasmid

#115186

Purpose

Lentiviral transduction and expression of PARK7E163K into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7E163K (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Mutation

p.E163K

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHAGE-C20orf24- C20orf24-3’UTR-VAR

Plasmid

#128509

Purpose

Lentiviral constitutive expression of C20orf24 under control of its native 3'UTR of human C20orf24 with variant from COX deficiency patient.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

RAB5IF (RAB5IF Human)

Use

Lentiviral

Tags

FLAG and HA tags

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

Plasmid

#113155

Purpose

An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

Plasmid

#113157

Purpose

An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat MANF

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

Plasmid

#174516

Purpose

Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

PylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon

Expression

Bacterial

Mutation

CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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