We narrowed to 17,590 results for: cript
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Plasmid#122313PurposeExpresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Rad21
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Rad21-R
Plasmid#122314PurposeExpresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Rad21
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ssrp1-L
Plasmid#122315PurposeExpresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ssrp1
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ssrp1-R
Plasmid#122316PurposeExpresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ssrp1
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9n-Nanog-L
Plasmid#122302PurposeExpresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Nanog (Nanog Synthetic)
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GreBdm-D41N
Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
ExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3Available SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
Tags6His-TEVExpressionBacterialPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
Tags6His-TEVExpressionBacterialMutationQ54APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT
Plasmid#61796PurposeLuciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutatedDepositorInsertmouse Ywhab 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT
Plasmid#61793PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutatedDepositorInsertmouse Xbp1 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT
Plasmid#61791PurposeLuciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutatedDepositorInsertmouse Smad5 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT
Plasmid#61790PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutatedDepositorInsertmouse Map3k1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseMutationmiR-200c MRE-1 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only