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pDONR223-ZNF768

Plasmid

#88524

Purpose

Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF768 (ZNF768 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-RacFRET-U6ac-ctrl guides

Plasmid

#168247

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1 SYS1

Plasmid

#175092

Purpose

SYS1-mEGFP can be transfected in mammalian cells

Depositor

Juan Bonifacino

Article

Ishida et al J Cell Biol. 2019 Nov 4;218(11):3681-369

Insert

SYS1 (SYS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68

Plasmid

#162650

Purpose

Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

Plasmid

#136546

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer8

Plasmid

#138580

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer4

Plasmid

#138576

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer1

Plasmid

#138573

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 enhancer (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

Plasmid

#128455

Purpose

lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-LSSmOrange

Plasmid

#118856

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-LSSmOrange

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry Tensin2

Plasmid

#105300

Purpose

To study localization and function of Tensin 2 with EGFP Tensin1

Depositor

Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin2 (TNS2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

lacks N-terminal C1 domain

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe NEO Venus

Plasmid

#98396

Purpose

Retroviral vector for constitutive Venus expression

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Venus

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE6

Plasmid

#79476

Purpose

sgRNA shuttle vector; module 1E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE8

Plasmid

#79463

Purpose

sgRNA shuttle vector; module 2E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB423

Plasmid

#68688

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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ERAS

Plasmid

#55656

Purpose

expression of mouse ERAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

ERAS (Eras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pBEG R2-iCreT2ALuc-L3 #BGV208

Plasmid

#48992

Purpose

Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Inserts

cre recombinase
Luciferase

Use

Gateway Cloning and Luciferase

Tags

IRES (weak)

Promoter

Unknown

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
SI11-Sema4c

Plasmid

#46301

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Sema4c mouse monoclonal antibody (sc:d411 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pWB-MF-IKKe

Plasmid

#44116

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

IKKe (IKBKE Human)

Use

Retroviral

Tags

Myristoylated Flad

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only