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pAAV-hSyn-DIO-mCherry-CW3SL

Plasmid

#223662

Purpose

Expresses mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Publication

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Publication

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_ChR2_mCherry

Plasmid

#213915

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_dTomato_nlsdTomato

Plasmid

#218792

Purpose

AAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Data Hub Report

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Pet28a-His-tag-BS2

Plasmid

#220347

Purpose

E. coli expression vector to purify BS2

Depositor

Bryan Dickinson

Publication

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

His tag

Expression

Bacterial

Available since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV-EF1a-BFP-W

Plasmid

#208757

Purpose

Lentiviral vector expressing BFP

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepII

Plasmid

#208617

Purpose

Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained

Depositor

Quanfu Ma

Type

Empty backbone

Tags

Strep II

Expression

Mammalian

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Publication

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GNb-FKBP

Plasmid

#128771

Purpose

Mammalian expression

Depositor

Colin Taylor

Publication

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FKBP

Expression

Mammalian

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJUMP49-2A(sfGFP)

Plasmid

#126964

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D(sfGFP)

Plasmid

#126963

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1C(sfGFP)

Plasmid

#126962

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1B(sfGFP)

Plasmid

#126961

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Publication

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-Sapphire

Plasmid

#103976

Purpose

Plasmid encoding Sapphire

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S72A, Y145F,…

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_HR_Prnp_3

Plasmid

#78621

Purpose

pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Publication

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

pX330_HR_Prnp_3

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

EFS and LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVk7-33G1-BioLHis

Plasmid

#58949

Purpose

backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

Depositor

Gavin Wright

Publication

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

6His tag and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP3 CKK

Plasmid

#59033

Purpose

expresses CKK domain of CAMSAP3 in E. coli

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CKK domain (CAMSAP3 Mouse)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only