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We narrowed to 57,324 results for: plasmid

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Showing: 10561 - 10580 of 57324 results

  1. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  2. qTAG-N-Solo-mStayGold-CETN2

    Plasmid
    #227292
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227292-plasmid-map-sequence-id-452065

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  3. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  4. qTAG-N-Blast-dTAG-LMNB1

    Plasmid
    #207776
    Purpose
    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207776-plasmid-map-sequence-id-416195

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  9. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  10. RAB27B

    Plasmid
    #25545
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB27B (RAB27B Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pRPA70-IRES-Ven-RPA32-IRES-RPA14

    Plasmid
    #211430
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Tags
    mVenus (on RPA32)
    Expression
    Mammalian
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211430-plasmid-map-sequence-id-420414

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  12. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Tags
    6xHis
    Expression
    Bacterial
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

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  13. cEF1a-GFP

    Plasmid
    #135671
    Purpose
    Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Available since
    March 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    135671-plasmid-map-sequence-id-325845

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  14. pENTR-L4R1-tetO2

    Plasmid
    #139525
    Purpose
    Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    tetO2 promoter
    Expression
    Mammalian
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139525-plasmid-map-sequence-id-274658

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  15. pENTR-L4R1-hEF1a

    Plasmid
    #139523
    Purpose
    Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    hEF1a promoter
    Expression
    Mammalian
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139523-plasmid-map-sequence-id-274622

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  16. pQXCINeo-LOX-BC

    Plasmid
    #134762
    Purpose
    a retroviral vector to express lysyl oxidase (derived from a cancer cell line)
    Depositor
    Philippe Clezardin, Martine Croset
    Insert
    LOX (LOX Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    R158Q relative to NP_002308.2
    Promoter
    CMV
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    134762-plasmid-map-sequence-id-264457

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  17. pSpot2

    Plasmid
    #117063
    Purpose
    For expression of proteins with C-terminal Spot-Tag in E. coli
    Depositor
    Felix Hartlepp
    Type
    Empty backbone
    Tags
    Spot-Tag
    Expression
    Bacterial
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117063-plasmid-map-sequence-id-226326

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  18. pSpot-Tag-Actin

    Plasmid
    #117060
    Purpose
    For mammalian expression of beta-actin with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    actin beta (ACTB Human)
    Tags
    Spot-Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117060-plasmid-map-sequence-id-224388

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  19. pOP3SU

    Plasmid
    #112599
    Purpose
    Used for expression of protein in E. coli as N-terminal Sumo tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    Sumo
    Expression
    Bacterial
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112599-plasmid-map-sequence-id-220209

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  20. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid
    #111152
    Purpose
    Chondrocyte specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Mutation
    wt
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111152-plasmid-map-sequence-id-211539

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Showing: 10561 - 10580 of 57324 results