We narrowed to 8,320 results for: Set

1. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL005

   Plasmid

   #233865

   Purpose

   MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA

   Plasmid

   #186411

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA

   Plasmid

   #186408

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP

   Plasmid

   #186412

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENT-L1-Kozak-NLSlacZ-stop-L2

   Plasmid

   #186358

   Purpose

   Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli, Synthetic)
   

   Use

   Expression of nuclear lacz reporter

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENT-L1-Kozak-HistoneH2B-L2

   Plasmid

   #186357

   Purpose

   Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Histone H2B (H2BC11 Human)
   

   Use

   Expression of a tagged nuclear marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus

   Plasmid

   #186409

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA

    Plasmid

    #186410

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 CARD8 ZUC

    Plasmid

    #169964

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 CARD8 FIIND L370G

    Plasmid

    #169970

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    L370G

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 CARD8 FIIND L368G

    Plasmid

    #169969

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    L368G

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 CARD8 PAM

    Plasmid

    #169975

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    PAM

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 CARD8 FIIND R394E

    Plasmid

    #169971

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    R394E

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 CARD8 Ub-S297

    Plasmid

    #169965

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Ub

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 CARD8 FIIND F405G

    Plasmid

    #169973

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    F405G

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221 CARD8 FIIND S297A

    Plasmid

    #169966

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HM3C NaVSp1 pET24b

    Plasmid

    #128088

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

    Depositor

    Dan Minor

    Article

    Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

    Insert

    NaVSp1p
    

    Use

    Tags

    3C Cleavage, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge