We narrowed to 3,796 results for: golden gate

1. (I) pGPD (J)_P3 (SBE111)

   Plasmid

   #194990

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (I) pGPD (J)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. (S) insD_ku70 (Q) (SBE139)

   Plasmid

   #187627

   Purpose

   the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (S) insD KU70 (Q)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. (P) insUP_ku70 (SBE138) (B)

   Plasmid

   #187626

   Purpose

   the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (P) insUP KU70 (B)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV10.K3.loxP

   Plasmid

   #63202

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV10.K5.loxP

   Plasmid

   #63204

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV10.HO3.loxP

   Plasmid

   #63205

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV10.HO6

   Plasmid

   #63211

   Purpose

   pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKS1576

   Plasmid

   #239524

   Purpose

   Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

   Depositor

   John Dueber

   Insert

   ScVba2p-mTurquoise2-2xCrk_SH3
   

   Expression

   Yeast

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAIP4_1UXZ

   Plasmid

   #234166

   Purpose

   Heterologous protein expression of cellulase B in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1UXZ
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHIE096 CMV Bxb1

    Plasmid

    #139252

    Purpose

    Encodes a Bxb1 recombinase for Landing Pad integration.

    Depositor

    Joshua Leonard

    Insert

    Bxb1 Recombinase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid

    #139254

    Purpose

    TRE3GV promoter and DsRedExpress2 in TUPV2

    Depositor

    Joshua Leonard

    Insert

    DsRedExpress2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM467 TRE3GV EYFP in TU3

    Plasmid

    #139255

    Purpose

    TRE3GV promoter and EYFP in TUPV3

    Depositor

    Joshua Leonard

    Insert

    EYFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS1338

    Plasmid

    #239511

    Purpose

    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    AtUTR1-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS1337

    Plasmid

    #239512

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKS1335

    Plasmid

    #239509

    Purpose

    Express Ymd8p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    ScYmd8p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS1336

    Plasmid

    #239510

    Purpose

    Express Hut1p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    ScHut1p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PACE_AP_Neg_pBADProm

    Plasmid

    #242141

    Purpose

    pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution

    Depositor

    Dominik Niopek

    Insert

    Gene III Negative
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PACE_AP_Pos_pBADProm

    Plasmid

    #242139

    Purpose

    pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution

    Depositor

    Dominik Niopek

    Insert

    Gene III
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS1466

    Plasmid

    #239516

    Purpose

    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScErg20p(F96W_N127W)-ePTS1
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKS1137

    Plasmid

    #239498

    Purpose

    Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-ePTS1
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge