We narrowed to 3,503 results for: golden gate

1. pPD1023 TRE3GV DsRedExpress2 in TU5

   Plasmid

   #139257

   Purpose

   TRE3GV promoter and DsRedExpress2 in TUPV5

   Depositor

   Joshua Leonard

   Insert

   DsRedExpress2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C77m

   Plasmid

   #120989

   Purpose

   MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   AmpR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. C78m

   Plasmid

   #120990

   Purpose

   MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ChlorR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. C80m

   Plasmid

   #120992

   Purpose

   MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mfLon protease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL11085_GG_Wheat_live_Cas9_for

   Plasmid

   #126881

   Purpose

   Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C86m

   Plasmid

   #120997

   Purpose

   MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   X174E
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C11m

   Plasmid

   #120959

   Purpose

   MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   Citrine
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. C5m

   Plasmid

   #120957

   Purpose

   MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).

   Depositor

   Richard Murray

   Insert

   mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C115m

   Plasmid

   #121014

   Purpose

   MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   TP901 integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b-BsaI

    Plasmid

    #233523

    Purpose

    Purification vector with BsaI cut sites for streamlined cloning

    Depositor

    Carl Denard

    Type

    Empty backbone

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS1170

    Plasmid

    #239504

    Purpose

    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-dPTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKS1171

    Plasmid

    #239505

    Purpose

    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-dPTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKS1517

    Plasmid

    #239518

    Purpose

    Express ClPT1(37-407) membrane enzyme cargo and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-ClPT1(37-407)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKS1520

    Plasmid

    #239520

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS1518

    Plasmid

    #239519

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS1138

    Plasmid

    #239499

    Purpose

    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS1139

    Plasmid

    #239500

    Purpose

    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide

    Depositor

    John Dueber

    Insert

    sfCherry3C(1-10)-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP4_2H7W

    Plasmid

    #234220

    Purpose

    Heterologous protein expression of chagasin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_2NT3

    Plasmid

    #234208

    Purpose

    Heterologous protein expression of response regulator homolog in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2NT3
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. U22m

    Plasmid

    #121025

    Purpose

    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

    Depositor

    Richard Murray

    Insert

    BCD6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge