We narrowed to 1,332 results for: pBAD

1. pBEL2196

   Plasmid

   #170400

   Purpose

   For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-717, replaced with 114-130)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL2197

   Plasmid

   #170401

   Purpose

   For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-716, replace with 347-360)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2198

   Plasmid

   #170402

   Purpose

   For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ702-716, replace with 580-600)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2251

   Plasmid

   #170403

   Purpose

   For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-717, replace with 225-249)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2252

   Plasmid

   #170404

   Purpose

   For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-716, replace with 455-479)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2253

   Plasmid

   #170405

   Purpose

   For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Use

   Tags

   8xHis2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ700-717, replace with 812-836)

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPR2_+10D+7U

   Plasmid

   #149393

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   7bp inserted upstream, and 10bp inserted downstre…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR2_+10D+10U

   Plasmid

   #149394

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted upstream and downstream of the IHFp…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNB201

    Plasmid

    #169112

    Purpose

    TESEC expression vector for Alr from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    alr (alr Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB202

    Plasmid

    #169113

    Purpose

    TESEC expression vector for mEGFP

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    mEGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVH009

    Plasmid

    #80399

    Purpose

    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain and N-terminal 3xFLAG tag, …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. C0080 (araC)_CD

    Plasmid

    #66029

    Purpose

    MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Transcription factor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSlb-wt

    Plasmid

    #60728

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

    Plasmid

    #153526

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#45

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#45
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSF-CjPglB-LpxE

    Plasmid

    #128389

    Purpose

    Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Inserts

    PglB oligosaccharylktransferase
    LpxE phosphatase
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and pBAD

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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