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pMEH365 Hspa8 HA

Plasmid

#168219

Purpose

Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Hspa8

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCis mCherry-SS Himar1 A7

Plasmid

#84687

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Use

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-FRB-tTA-Myc

Plasmid

#177911

Purpose

FRB-tTA-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-tTA

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TS245_pNKI-2-13_EGFP-DPN7

Plasmid

#200719

Purpose

producing Methyl Tracer EGFP protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

eGFP-DPN7

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-empty-pgk-hph

Plasmid

#48753

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only
pHHYTK113

Plasmid

#200144

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

sfGFP1-10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.B

Plasmid

#201005

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

TagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-HA

Plasmid

#198296

Purpose

Produces PVCs retargeted with HA tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-HA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pIEx4-Cre

Plasmid

#198280

Purpose

Expresses Cre in insect cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pIEx4-Cre

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Use

Tags

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc13

Plasmid

#198283

Purpose

Produces PVCs lacking tail fibers

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc13

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEM3 = ncs-1p::nCre

Plasmid

#24034

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

ncs-1 (ncs-1 Nematode)

Use

Cre/Lox

Tags

nCre

Expression

Worm

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-OT

Plasmid

#184596

Purpose

For expression of a fluorescent sensor for oxytocin in mammalian cells

Depositor

Daisuke Ino, Hiroshi Hibino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRBBm59

Plasmid

#48115

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Use

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMEH275 NLS dTomato

Plasmid

#168210

Purpose

Mammalian expression of nuclear-localized dTomato

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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