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pSALECT-Efnb2

Plasmid

#59707

Purpose

pSALECT with Efnb2 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Efnb2 (Efnb2 Mouse)

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Gal4(1-94) HSF ABC Flag ph

Plasmid

#1947

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

Gal4 DBD and flag

Expression

Mammalian

Mutation

phenylalanine mutant (F418, F492, F500 A)

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pUPD2_EBSn-mini35S (A1-B1)

Plasmid

#254062

Purpose

Contains synthetic promoter EBSn-mini35S

Depositor

Jose Alonso, Anna Stepanova

Article

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn-mini35S (A1-B1)

Use

Synthetic Biology

Expression

Plant

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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Modified pYTK075

Plasmid

#254020

Purpose

A part plasmid designed to use the yeast selective marker (LEU2 gene) as a Level 1 TU fragment.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LEU2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-ΔHsFBXO28-Myc-MBP-HA

Plasmid

#228946

Purpose

Expression ΔHsFBXO28-Myc (lacking F-box domain) and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

ΔHsFBXO28 (FBXO28 Human)

Tags

myc

Expression

Bacterial

Mutation

deletion of the F-box domain

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLux*R1cI*

Plasmid

#250891

Purpose

Frame shifted cI protein expressed under PLux* promoter; wild type cI expressed under PLLacO1 promoter; component of BNeuAS6; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Wild type cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein; PLux* = Point mut…

Promoter

PLLacO1 and PLux*

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pZK6

Plasmid

#248911

Purpose

Expresses yeast Isc10 fused to maltose binding protein in bacteria

Depositor

Edward Winter

Article

Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:

Insert

Isc10 (ISC10 Budding Yeast, Sequence is optimized for bacteria to produce the Isc10 protein from yeast (protein sequence accession below))

Tags

maltose binding protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-chKlacRS-IPYE

Plasmid

#232160

Purpose

A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

chKlacRS-IPYE

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_022_aac6-aph2-marker

Plasmid

#235898

Purpose

aac6-aph2 tobramycin resistance gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

aac6-aph2-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_001_psbH_5'Hom_Cr

Plasmid

#235784

Purpose

5' Homology psbH in the M1 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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