We narrowed to 56,849 results for: plasmid
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Plasmid#227282PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.DepositorInsertsgRNA Targeting N-terminus of CENPA (CENPA Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK
Plasmid#126928PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2A(sfGFP)
Plasmid#126964PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
ExpressionBacterialAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1D(sfGFP)
Plasmid#126963PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1C(sfGFP)
Plasmid#126962PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1B(sfGFP)
Plasmid#126961PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTagsStrep II (STR); TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HISDHFR
Plasmid#63935PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kv2.1/RBG4
Plasmid#196947PurposePlasmid expressing an antigen targeting Kv2.1 (RBG4)DepositorInsertKv2.1 (Kcnb1 Rat)
ExpressionMammalianAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nxn-1 beta Flag/pCMV
Plasmid#197339PurposePlasmid expressing an antigen targeting Neurexin 1betaDepositorAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_306-ccdB-zeo
Plasmid#201134PurposepLEX_306 plasmid with zeo markerDepositorTypeEmpty backboneExpressionMammalianPromoterSV40Available SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-EBFP2
Plasmid#201137PurposepLEX_307 plasmid with EBFP2 markerDepositorTypeEmpty backboneExpressionMammalianPromoterEF-1αAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
NGL-1-myc/pEGFP-N1
Plasmid#197314PurposePlasmid expressing an antigen targeting Neuroligin-1DepositorInsertNeuroligin-1 (Nlgn1 Rat)
ExpressionMammalianAvailable SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK082 desmaMCS:minprommCherry,exorh:mCerulean
Plasmid#200015PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCeruleanDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-eGFP
Plasmid#193023PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInserteGFP
UseAAVExpressionMammalianPromoterPGKAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …MutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide
Plasmid#186993PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, non-targeting crRNA guide
UseAAVMutations1006-gggs-d1221 deletionAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only