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pNC-TRV2

Plasmid

#193402

Purpose

Nimble cloning-compatible vector for virus-induced gene silencing

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Expression

Worm

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED1

Plasmid

#193426

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PMT-mScarlet

Plasmid

#203779

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mScarlet

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mTurquoise2_2

Plasmid

#203776

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2 dimer

Expression

Mammalian

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mTurquoise2

Plasmid

#203775

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR082

Plasmid

#188553

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR082

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pUG-57

Plasmid

#104714

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

Ub10 (UBQ10 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PNCA-mCherry

Plasmid

#67790

Purpose

Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

Insert

MMLV-LTR-mCherry

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
TOM20-SNAPf-mTurquoise2

Plasmid

#197496

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the mitochondria.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED4

Plasmid

#193429

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-mScl

Plasmid

#64895

Purpose

Lentiviral expression of Flag-tagged murine transcription factor Scl

Depositor

Georges Lacaud

Article

Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

Insert

Scl

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRpoH-Wasabi

Plasmid

#86107

Purpose

mWasabi reporter gene driven by RpoH promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pRpoH

Available Since

Feb. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ACE16-NLS

Plasmid

#210727

Purpose

NIR light-activated intein-based gene expression system (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

ACE16-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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