We narrowed to 27,031 results for: gfp gene

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pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227921

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227897

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

NCLN homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227903

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3328 PB-sfGFP-WPRE-target

Plasmid

#226676

Purpose

PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

sfGFP-WPRE-site3target

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_eGFP-pScaE

Plasmid

#228826

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaE

Tags

eGFP

Expression

Mammalian

Mutation

aa 49-494

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC

Plasmid

#228824

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-P2A-GFP

Plasmid

#229405

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaA

Plasmid

#228823

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaA

Tags

eGFP

Expression

Mammalian

Mutation

aa 25-1216

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

Plasmid

#194878

Purpose

Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

Plasmid

#194879

Purpose

Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUA-IRES-mCherry

Plasmid

#222116

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AUA start codon

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGC-IRES-mCherry

Plasmid

#222119

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AGC start codon

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L, L438K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgGFP

Plasmid

#213169

Purpose

Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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