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Plasmid#203541PurposegRNA expression in astrocytesDepositorInsertNLS-GFP, 2x U6-RNA
UseAAVExpressionMammalianAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
ExpressionBacterialPromoterpBADAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_Txe/Axe
Plasmid#229147PurposeToxin-Antitoxin (Txe/Axe)DepositorInsertmCherry, Txe/Axe
ExpressionBacterialPromoterPtlpA (mCherry), Txe/Axe (Native)Available sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3241 PB-AtoBpegRNA-mCherry
Plasmid#226666PurposePiggyBac cargo vector encoding A to B pegRNA marked with mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtoB pegRNA
UseCRISPRExpressionMammalianAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD86
Plasmid#223598PurposeLentiviral expression of human CD86 with SpyTag003 in mammalian cellsDepositorAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
V5-BS2-SENP2 (Nuclear Pore)
Plasmid#220345PurposeMammalian expression vector BS2 in nuclear poreDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBS2
TagsSENP2 (nuclear pore localized protein) and V5 tagExpressionMammalianPromoterCMVAvailable sinceJune 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTNR1A-DuET
Plasmid#213348PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTNR1A (MTNR1A Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinD-IRES-Puro
Plasmid#213114PurposeExpression of untagged MinD in mammalian cellsDepositorAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralMutationG183S mutation in Galpha-i3 (renders it RGS insen…Available sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS1928
Plasmid#205967PurposeJAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)DepositorInsertCas8-HNH all proteins, with His-tagged Cas8
Tags14xHis on Cas8ExpressionBacterialPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV_PGK_LTB4R
Plasmid#208087PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R.DepositorAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP-CAAX
Plasmid#208685PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent mannerDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseAAVPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb80
Plasmid#205519PurposeNuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.DepositorInsertnuc-Nb80
TagsNuclear localization signal (NLS) and mCherryExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GPI
Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD58 c-term (CD58 Human)
TagsmEos3.2ExpressionMammalianAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
ExpressionBacterialPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only