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pDONR221-ZNF665

Plasmid

#101448

Purpose

Donor Vector containing ZNF665 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF665 (ZNF665 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A870L

Plasmid

#174797

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A870L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A870L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-K893I

Plasmid

#174799

Purpose

Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-K893I (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

K893I, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF879

Plasmid

#101623

Purpose

Donor Vector containing ZNF879 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF879 (ZNF879 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF646

Plasmid

#101625

Purpose

Donor Vector containing ZNF646 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF646 (ZNF646 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF735

Plasmid

#101595

Purpose

Donor Vector containing ZNF735 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF735 (ZNF735 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF587B

Plasmid

#101621

Purpose

Donor Vector containing ZNF587B transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF587B (ZNF587B Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF222

Plasmid

#101622

Purpose

Donor Vector containing ZNF222 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF222 (ZNF222 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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