We narrowed to 56,849 results for: plasmid

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Article

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Expression

Plant

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti4-Puro-H19

Plasmid

#200835

Purpose

Lentiviral vector expressing human long non-coding RNA H19

Depositor

Reinier Boon

Article

Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

Insert

Human long non-coding RNA H19

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Expression

Bacterial

Promoter

glnS' and lambda(ts) and proK

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FOSL2-P2A-mCherry

Plasmid

#203860

Purpose

Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

FOSL2 (FOSL2 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET-His6MBP-A3A-Gly6His6

Plasmid

#187822

Purpose

Expression human APOBEC3A in E.coli.

Depositor

Hao Wu

Article

Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

Insert

APOBEC3A (APOBEC3A Human)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

none

Promoter

T7 promoter (on the backbone)

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9_sgAAVS1

Plasmid

#199213

Purpose

All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

AAVS1-gRNA

Use

CRISPR

Promoter

human U6

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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SupD v2

Plasmid

#198598

Purpose

Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SupD

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC4_S

Plasmid

#147455

Purpose

Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsEDC4 (EDC4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR4A1

Plasmid

#192925

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR4A1 (NR4A1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

Plasmid

#195503

Purpose

SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)

Use

CRISPR; Donor vector

Tags

T2A-H2B-mCitrine

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12Max

Plasmid

#195334

Purpose

vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized Cas12Max

Tags

3xFlag

Expression

Mammalian

Mutation

N243R

Promoter

CBh, CMV, hU6

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMF-FU_TetO_GW_PPARG2

Plasmid

#193289

Purpose

Lentiviral construct that expresses a dox-inducible human PPARG2

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

PPARG2 (PPARG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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dTAG-IRF8

Plasmid

#190620

Purpose

Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

IRF8 (IRF8 Human)

Use

Lentiviral

Tags

FKBP12_G36V

Expression

Mammalian

Mutation

synonymous mutation for sgRNA resistance for sgIR…

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_ATG4B_BamHI_NotI

Plasmid

#190862

Purpose

Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

ATG4B (ATG4B Human)

Tags

GST, Thrombin Cleavage site

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-HALO-CTERM-GFP

Plasmid

#185767

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-AID-NTERM-GFP

Plasmid

#185773

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pU1_000

Plasmid

#186773

Purpose

* Engineered U1 snRNA plasmid for insertion of guide sequences.

Depositor

Eugene Yeo

Article

Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U1 Promoter

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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