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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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164,826 results

Showing: 10701 - 10720 of 164826 results

  1. pCMVMagFRET-1

    Plasmid
    #50742
    Purpose
    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Insert
    gene encoding for MagFRET-1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50742-plasmid-map-sequence-id-327713

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  3. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32442-plasmid-map-sequence-id-117095

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  10. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid
    #178429
    Purpose
    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    EGFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178429-plasmid-map-sequence-id-354737

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  12. pUBC-Pfv-Sapphire_PURO

    Plasmid
    #203651
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203651-plasmid-map-sequence-id-402481

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  13. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Promoter
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  15. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    modified lac
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59449-plasmid-map-sequence-id-92571

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  16. pICE-HA-MRE11-WT

    Plasmid
    #82033
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82033-plasmid-map-sequence-id-155383

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  20. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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Showing: 10701 - 10720 of 164826 results