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pFX-mito-EYFP

Plasmid

#174182

Purpose

Express EYFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EYFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 WT

Plasmid

#27451

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa, GST, and His6

Expression

Bacterial

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]

Plasmid

#247263

Purpose

Express ISCro4 recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2922 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-mCherry

Plasmid

#80946

Purpose

human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

mCherry

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLAMP1-emiRFP670

Plasmid

#136570

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LAMP1-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-LK03

Plasmid

#206512

Purpose

AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

Insert

LK03 capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233353

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-mito-mKate2

Plasmid

#174178

Purpose

Express mKate2 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pTK88_GFP-Anillin

Plasmid

#46354

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Anillin (ANLN Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

a1854 is changed to c to disrupt XhoI site withou…

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX puro TRIM21-GFP

Plasmid

#116941

Purpose

Expression of TRIM21-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 - GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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