Addgene

We narrowed to 11,038 results for: Hal

Showing: 2121 - 2160 of 11038 results

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pICH45234

Plasmid

#50276

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38430, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45214

Plasmid

#50278

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, LHB1B1 (AT2g34430, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

David Schaffer, John Dueber

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

David Schaffer, John Dueber

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pME-lamp1

Plasmid

#109559

Purpose

Multisite gateway vector for 5' tagging with lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab7a

Plasmid

#109723

Purpose

Multisite gateway vector for 3' tagging with rab7a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7a (rab7a Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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TRPML1-YFP

Plasmid

#18826

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMN-334

Plasmid

#195468

Purpose

Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab32b

Plasmid

#140885

Purpose

Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab32b (rab32b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA3

Plasmid

#26679

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 3 (KPNA3 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pMN-333

Plasmid

#195467

Purpose

Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-332

Plasmid

#195466

Purpose

Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Use

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Promoter

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA5

Plasmid

#26681

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 5 (KPNA5 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ-176

Plasmid

#195424

Purpose

Transient, constitutive expression of synZiFTR: ZF1-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-009

Plasmid

#195413

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-TET3G-T2A-Puro

Plasmid

#158067

Purpose

Encodes the Tet3G transactivator and puromycin resistance.

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

pCAG-TET3G-T2A-Puro

Use

AAV

Tags

puromycin reistance gene

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ-347

Plasmid

#195458

Purpose

Lentiviral Integratable, constitutive expression of synZiFTR: ZF10-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - 3XFLAG - NLS - ZF1 - p65

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML1-CFP

Plasmid

#18827

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tK)

Plasmid

#109488

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tK)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML1-HA

Plasmid

#18825

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-smyhc1

Plasmid

#126748

Purpose

Zebrafish slow myosin heavy chain promoter. Clone 667

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

smyhc1 (smyhc1 Zebrafish)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT/TO/FLAG/HA-DEST TNRC6A

Plasmid

#19883

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG/HA-DGCR8

Plasmid

#10921

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

DGCR8 (DGCR8 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
15x-QUAS-dTomato-T2A-GCaMP6s

Plasmid

#130666

Purpose

QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

15xQUAS-dTomato-T2A-GCaMP6s

Use

Ae. aegypti expression

Tags

Expression

Insect

Mutation

Promoter

15xQUAS-TATA

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLP710

Plasmid

#203740

Purpose

Integration of OsTIR1-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Expression

Mutation

Promoter

ACT1 (C. albicans)

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-nCas9n-P2A-TagBFP2-pA

Plasmid

#176026

Purpose

Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n-P2A-TagBFP2

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX GFP Rac G12V

Plasmid

#14567

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

Rac1 G12V (RAC1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

G12V

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

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