We narrowed to 1,212 results for: Ncl
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Plasmid#71397PurposeHomologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
TagsSV-40 GFP insertion in TERT promotorPromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-DMD-Donor
Plasmid#60605PurposeKnock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.DepositorInsertHygromycin resistant gene
UseKnock-in donor vectorExpressionMammalianAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGMutationChanged F79 to GAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC-CLEC4E
Plasmid#172412PurposeMAC-tagged gene expressionDepositorInsertC-type lectin domain family 4 member E (CLEC4E Human)
TagsMAC-tagExpressionMammalianPromoterCMV promoterAvailable SinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos3.2FP
Plasmid#203757PurposeEncodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)
Plasmid#160641PurposeModule for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.DepositorInsertERLexABDGal4AD / PhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-TIR1-NOSt
Plasmid#108545PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 G domain
Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianPromoterSFFVAvailable SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS
Plasmid#241915PurposeZebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASM1Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pH-EF1α-DualPE
Plasmid#236417PurposeFor plant prime editing including large DNA fragment editing in Solanum lycopersicum or other dicotyledonsDepositorInsertEF1α promoter
UseCRISPRExpressionPlantAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pH-2×35S-DualPE
Plasmid#236416PurposeFor plant prime editing including large DNA fragment editing in Nicotiana benthamiana or other dicotyledonsDepositorInserts2×35S promoter
hygromycin-resistance gene
UseCRISPRMutationDetailed in manuscriptAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
BtARR1 Cys-less
Plasmid#236274PurposeBacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATGDepositorInsertarrestin-1 (SAG Bovine)
ExpressionBacterialMutationC63A, C128S, C143A, inserted GGT codon after star…PromoterlacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC175_HBA1reg(synEPOR)
Plasmid#232413PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B G120A-TdLanYFP
Plasmid#228561PurposeEncodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
eft-3p:act-5(ptc):tbb2 3' UTR
Plasmid#202331PurposeThe ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.DepositorInsertseft-3 promoter
act-5(ptc)
tbb-2 3' UTR
Tagsbase 238 C -> A mutation causing a PTC at codo…ExpressionWormPromoterThis is a promoter sequence for C. elegans eft-3,…Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC4 CD H976Y
Plasmid#224250PurposeExpresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coliDepositorInsertKDAC4 (HDAC4 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc5.1B-lambdaN-HA-DmGW182-V5His6_H
Plasmid#146499PurposeInsect Expression of DmGW182DepositorInsertDmGW182 (gw Fly)
ExpressionInsectMutationTwo non silent mutations V704A and N799S and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only