We narrowed to 24,460 results for: Spr

1. LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

   Plasmid

   #209923

   Purpose

   A knock-out vector for the dog CTNND1

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   A gRNA targeting the dog CTNND1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-CASPR/Neurexin IV [K65/35R-rat IgG2a]

   Plasmid

   #222178

   Purpose

   Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

   Plasmid

   #221447

   Purpose

   Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   SPR degron: hGEM (1-110) fragment that is fused t…

   Promoter

   CMV

   Available Since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN

   Plasmid

   #209786

   Purpose

   Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   TNT4, TadA8e, SpRY N, inteinN
   

   Use

   AAV

   Promoter

   TNT4

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124283

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

6. Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124285

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

7. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124284

   Purpose

   sgRNA against human Thy-1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75246

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75247

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid

    #62580

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

    Plasmid

    #62579

    Purpose

    Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid

    #62581

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid

    #138148

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138145

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138146

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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