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We narrowed to 172,994 results for: addgene

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Showing: 1061 - 1080 of 172994 results

  1. pLFYa

    Plasmid
    #243803
    Purpose
    Dropout vector for genomic integration into yeast strain LFYa. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains the attP site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243803-plasmid-map-sequence-id-487915

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  2. pLFYalpha

    Plasmid
    #243804
    Purpose
    Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243804-plasmid-map-sequence-id-487916

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  3. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  4. LentiCRISPRv2-ACTB-C4

    Plasmid
    #229849
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229849-plasmid-map-sequence-id-462154

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  5. pX330-U6-MYC-Guide-hSpCas9

    Plasmid
    #228574
    Purpose
    Expression of human MYC gRNA; CRISPR mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    MYC gRNA (MYC Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228574-plasmid-map-sequence-id-457356

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  6. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  7. pDK362

    Plasmid
    #134194
    Purpose
    Retroviral plasmid with SKAP insert
    Depositor
    Iain Cheeseman
    Insert
    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    Use
    Retroviral
    Tags
    LAP (GFP)
    Expression
    Mammalian
    Mutation
    aa 166-316 with G5 and Jens siRNA hardening
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    134194-plasmid-map-sequence-id-272444

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  8. pBRCA2.1.0-gDNA

    Plasmid
    #132448
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    BRCA2 (BRCA2 Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132448-plasmid-map-sequence-id-266805

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  9. pKLF6.1.0-gDNA

    Plasmid
    #132435
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    KLF6 (KLF6 Human)
    Use
    CRISPR
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132435-plasmid-map-sequence-id-266217

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  10. pZNF462.1.0-gDNA

    Plasmid
    #112476
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462
    Depositor
    Kevin White
    Insert
    ZNF462 (ZNF462 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112476-plasmid-map-sequence-id-230510

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  11. pTBPL1.1.0-gDNA

    Plasmid
    #112412
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1
    Depositor
    Kevin White
    Insert
    TBPL1 (TBPL1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112412-plasmid-map-sequence-id-230271

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  12. pSMAD3.1.0-gDNA

    Plasmid
    #112407
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3
    Depositor
    Kevin White
    Insert
    SMAD3 (SMAD3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112407-plasmid-map-sequence-id-230266

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  13. WNK2 (WNK2A-c003)

    Plasmid
    #110250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK2 (WNK2 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110250-plasmid-map-sequence-id-212284

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  14. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101880-plasmid-map-sequence-id-194708

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  15. pPyCAG-Nanog(Q124A)-IP

    Plasmid
    #62993
    Purpose
    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q124A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 124 (125 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62993-plasmid-map-sequence-id-107891

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  16. pPyCAG-Nanog(M125A)-IP

    Plasmid
    #62994
    Purpose
    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog M125A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Methionine 125 (126 AA in Mouse Protein) …
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62994-plasmid-map-sequence-id-107893

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  17. pPyCAG-Nanog(Q138A)-IP

    Plasmid
    #62997
    Purpose
    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q138A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 138 (139 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62997-plasmid-map-sequence-id-104599

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  18. pPyCAG-Nanog(T141A)-IP

    Plasmid
    #62999
    Purpose
    Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T141A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Threonine 141 (142 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62999-plasmid-map-sequence-id-104602

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  19. pPyCAG-Nanog(Q144A)-IP

    Plasmid
    #63000
    Purpose
    Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q144A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 144 (145 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63000-plasmid-map-sequence-id-104604

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  20. pPyCAG-Nanog(N145A)-IP

    Plasmid
    #63001
    Purpose
    Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog N145A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Asparagine 145 (146 AA in Mouse Protein) …
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63001-plasmid-map-sequence-id-104605

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Showing: 1061 - 1080 of 172994 results