We narrowed to 159,238 results for: addgene

1. pBS35

   Plasmid

   #83797

   Purpose

   Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-tkd2MetGFP

   Plasmid

   #37562

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only contains cytoplasmic domain amino acids L115…

   Promoter

   CMV

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIM33 (TRIM33B-c100)

   Plasmid

   #98243

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TRIM33B (TRIM33 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Amber C1

   Plasmid

   #27797

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   mAmber C1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS4

   Plasmid

   #83793

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4QQ6

   Plasmid

   #61946

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SMN1:JMC047-F11:C221089 (SMN1 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. CYP11A1 (3N9Y)

   Plasmid

   #28119

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pBS10

   Plasmid

   #83795

   Purpose

   Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPD95.77-wVenus

   Plasmid

   #37465

   Depositor

   Hitoshi Sawa

   Article

   Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBPcyto-TEV-BseRI

    Plasmid

    #157739

    Purpose

    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Use

    Tags

    His10 + MBP (cytoplasmic expression; no signal se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBES2

    Plasmid

    #83800

    Purpose

    Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Venus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6D1T (MBD1)

    Plasmid

    #119966

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD1 (MBD1 Human)
    

    Use

    Tags

    GSTtag, 6xHIS and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pUPD2:35S-TMV-Rep-MP

    Plasmid

    #106217

    Purpose

    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

    Depositor

    Tomáš Moravec

    Insert

    Infectious clone of Tobacco mosaic virus, 5' part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6WNX

    Plasmid

    #162270

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    FBXW11 (FBXW11 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. BAZ2B

    Plasmid

    #73229

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BAZ2B (BAZ2B Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00439:Bromodomain 1871-1955

    Promoter

    T7

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKG204

    Plasmid

    #63078

    Purpose

    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    V151I mutation compared to GenBank reference sequ…

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVD621

    Plasmid

    #66162

    Purpose

    to make a ctxB probe

    Depositor

    James Kaper

    Article

    Michalski et al Infect Immun. 1993 Oct;61(10):4462-8.

    Insert

    ctxB
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. WDR39

    Plasmid

    #25308

    Purpose

    Expresses His-tagged WDR39 (CIAO1) in bacteria

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR39_1 (CIAO1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pKG326

    Plasmid

    #69753

    Purpose

    Retroviral expression of CENP-T aa 1-531

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-531 (CENPT Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    E.coli optimized sequence; A216T and G459R mutati…

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. 6XKC

    Plasmid

    #162271

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    FEM1C (FEM1C Human)
    

    Use

    Tags

    6xHis, GST and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    GST-F:tgaagctatcccacaaattg

    Available since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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