We narrowed to 2,816 results for: cdna expression plasmid

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pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL delta K5S

Plasmid

#34594

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127315

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

Plasmid

#158604

Purpose

Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

Plasmid

#127329

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG

Plasmid

#127328

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

Plasmid

#225586

Purpose

Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

F8.2(S54L+T127A)-T2A-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1

Plasmid

#158775

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD_1-270

Plasmid

#218229

Purpose

Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 1-270

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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