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Plasmid#177074PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsert8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-V4
Plasmid#173794PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJOG250
Plasmid#80582Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIDepositorInsertspnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-YFP
Plasmid#62630Purposemammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationYFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-z ONE-GO
Plasmid#189734PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-YFP
Plasmid#62634Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationYFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationI250 (silent mutation in YFP)PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationL342M (present in YFP but no functional consequen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtPuc3_diaCas9_sgRNA
Plasmid#109219PurposeEpisome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutumDepositorInsertsLHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterLHCF1 terminator, LHCF2 promoter, U6 3' regi…Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7
Plasmid#62276Purposemammalian expression of human Alpha7 (CHRNA7)DepositorInsertCHRNA7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-CMV-Galpha-12 ONE-GO
Plasmid#189739PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-13 ONE-GO
Plasmid#204337PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-13 ONE-GO (GNA13 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsIRFP and LEXYExpressionInsectMutationPromotermaternal tubulinAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-myc-Itch (C822S)/2860
Plasmid#11428DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationcysteine at 822 mutated to serinePromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEVA1
Plasmid#58953PurposeContains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.DepositorInsertVAR1u, VAR1 recoded for expression from the nucleus
UseYeast ecoli shuttle vectorTagspreCox4 mitochondrial matrix targeting signalExpressionYeastMutationSynthetic gene in standard genetic codePromoterADH1Available sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-mGFP
Plasmid#62636Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsmEGFP inserted in M3-M4 loopExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A ca…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only