We narrowed to 1,209 results for: proc.2

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pAAV-nEF-Coff/Fon taCasp3-TEVp

Plasmid

#256275

Purpose

Expresses autocleavable Caspase3 in Flp expressing and Cre non-expressing cells

Depositor

Jean-François Poulin

Publication

Bolduc et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

INTRSECT taCasp3-TEVp

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Mutation

None

Promoter

nEF

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff taCasp3-TEVp

Plasmid

#256274

Purpose

Expresses autocleavable Caspase3 in Cre expressing and Flp non-expressing cells

Depositor

Jean-François Poulin

Publication

Bolduc et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

INTRSECT taCasp3-TEVp

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Mutation

None

Promoter

nEF

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pNoTag-DGCR8

Plasmid

#164694

Purpose

Express DGCR8 without tag in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

DGCR8 (DGCR8 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has service

AAV2

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Mutation

G144A, Y145A, G146A

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir125-CMV2-mir1

Plasmid

#164668

Purpose

Express miR-125a and miR-1-1 in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-125a (MIR125A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-128-1

Plasmid

#46675

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-128-1 (MIR128-1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-mir144~451 EF1Alpha-puro-T2A-BFP

Plasmid

#164793

Purpose

Express miR-144~451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1

Plasmid

#46672

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-92a-1 (MIR92A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Publication

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Publication

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Tags

cfSBFP2

Expression

Mammalian

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Publication

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R3tdTomatoi2

Plasmid

#185969

Purpose

Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

Depositor

Sangram Bagh

Publication

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJOE4847.2

Plasmid

#135086

Purpose

rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Publication

Motejadded et al Biotechnol Lett. 2009 Apr;31(4):543-9. d

Insert

MalE-6xHis-Ulp1

Expression

Bacterial

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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