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pLenti CMV TRE3G Neo GFP-Progerin

Plasmid

#118710

Purpose

Lentiviral TET-ON inducible GFP-Progerin

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTNS3

Plasmid

#63127

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

Type

Empty backbone

Expression

Bacterial

Promoter

P1 and Ptac

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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miniTurbo-V5_pRS415

Plasmid

#107168

Purpose

expresses V5-tagged miniTurbo in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Yeast

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-SypHer3s-dmito

Plasmid

#108119

Purpose

Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s-dmito

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-K-GECO1

Plasmid

#105865

Purpose

Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Expression

Mammalian

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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