We narrowed to 11,595 results for: Emb;
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Plasmid#228531PurposeF-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)
Plasmid#228533PurposeA fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls BFP FLAG WPRE bGH pA (JDW 484)
Plasmid#224539PurposeA Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleusDepositorInsertnls-mTagBFP2-FLAG_WPRE-bGH-pA
UseGateway CloningAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHsp70(K71E)-GFP
Plasmid#228196Purposeexpressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tagDepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito(OM)-deltaCKAR
Plasmid#222431PurposeFRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.DepositorInsertMitochondrial outer membrane delta-selective C kinase activity reporter
TagsCFP and YFPExpressionMammalianAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)
Plasmid#227620PurposeOverexpression of SLC25A46 with R247A and R347A mutations in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationR257A,R347APromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB53
Plasmid#226309PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-3
ExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
Plasmid#225490PurposeNeuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseNeuronal expressionExpressionMammalianPromoterhSynAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)
Plasmid#224524PurposeA Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassetteDepositorInsertrox-3xNLS-mCherry-V5-2xSTOP-rox
UseGateway CloningAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PDL1-LgBiT
Plasmid#223619PurposeLentiviral expression of human PD-L1 with LgBiT tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-SNAP
Plasmid#223612PurposeLentiviral expression of human CD80 with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(SGGG)
Plasmid#223607PurposeLentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPMutationMYPPPY mutated to SGGGAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
TagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
TagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…Available SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
TagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361AAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1 5W
Plasmid#213629PurposeBacterial expression of SUMO-tagged rat FL-syt1 5WDepositorInsertFL-syt1 5W (Syt1 Rat)
TagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only