We narrowed to 63,769 results for: cloning
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Plasmid#163797PurposeExpresses Sa-SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only
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pSW505 - pOp4:3xGFP-NLS
Plasmid#116005Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
Tagslinker-NLSExpressionPlantAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionWorm and YeastAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL
Plasmid#83342Purposefor Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB409
Plasmid#74803PurposeGateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL8215 (dCas12f–σE (sigmaE) system, entry vector)
Plasmid#254272PurposeEncodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloningDepositorInsertsdCas12f
sigma E
UseCRISPRExpressionBacterialPromoterJ23105Available sinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJRA107
Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22D
Plasmid#91136PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantMutationD10A, H840AAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iBlast-L3 #BGV204
Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway CloningTagsIRES (weak)PromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorPublicationTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
TagsHis-tagExpressionBacterialAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_frD2 ΔE6 3* Δ7a/H1 WSN
Plasmid#253149PurposeFor recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (WSN) replaces orf7aDepositorInsertCoV-2 frD2 with Influenza H1 WSN
ExpressionBacterialAvailable sinceOct. 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_frD2 ΔE6 3* Δ7a/H1 PR8
Plasmid#253148PurposeFor recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf7aDepositorInsertCoV-2 frD2 with Influenza H1 PR8
ExpressionBacterialAvailable sinceOct. 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_ frD_ΔE3/F Δ67 DS Cav1 ΔCT
Plasmid#253163PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; pre-fusion stabilized RSV F replaces orf3a and envelopeDepositorInsertCoV-2 frD with RSV preF
ExpressionBacterialAvailable sinceOct. 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_frD_S Omicron BA.4/5
Plasmid#253118PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.4/5 spike; chimericDepositorInsertSARS-CoV-2 Omicron BA.4/5 spike
ExpressionBacterialAvailable sinceSept. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2 frD_ΔrE/GFP
Plasmid#253120PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA). GFP is inserted in the locus of envelopeDepositorInsertFragment D2 SARS-CoV-2 ΔE-GFP
ExpressionBacterialAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_frD ΔS/VSVG ΔE67 3*
Plasmid#253173PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces spikeDepositorInsertCoV-2 frD with VSV-G
ExpressionBacterialAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a
Plasmid#253172PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelopeDepositorInsertCoV-2 frD with VSV-G
ExpressionBacterialAvailable sinceMay 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a
Plasmid#253169PurposeFor recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelopeDepositorInsertCoV-2 frD with Influenza N1 PR8
ExpressionBacterialAvailable sinceMay 13, 2026AvailabilityAcademic Institutions and Nonprofits only