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pHK401

Plasmid

#235482

Purpose

Repression of gene VIII by Plux/tet hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK201

Plasmid

#235481

Purpose

Repression of gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK009

Plasmid

#235459

Purpose

NOR gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOR gate

Use

Synthetic Biology

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.3QS

Plasmid

#235485

Purpose

inducible NOT gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.23

Plasmid

#235484

Purpose

dual NOT gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-Nat

Plasmid

#236485

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-TO-g0 (FLP-IN)

Plasmid

#236120

Purpose

Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

g0

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-Nat

Plasmid

#236488

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-Nat

Plasmid

#236491

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Nat

Plasmid

#236479

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PX458-eBFP2-sgRNA_CTCF_ZF1

Plasmid

#233090

Purpose

Expression vector for a sgRNA against the mouse CTCF ZF1 region and SpCas9-T2A-eBFP2.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

spCas9-T2A-eBFP2 (Ctcf )

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pS4

Plasmid

#231613

Purpose

L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals

Depositor

Hans Christopher Bernstein

Article

Chan et al Biodes Res. 2023 Aug 16;5:0016. doi: 10.

Insert

Ptet-AraC-mKate2-Pbad-TetR-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pbad/Ptet

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

Plasmid

#233240

Purpose

Parallel coiled-coil with (GGS)3 linker to prone condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)3-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

Plasmid

#233241

Purpose

Parallel coiled-coil with (GGS)12 linker to prevent condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)12-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_RNF157_SOX9scr_neg0str

Plasmid

#232373

Purpose

RNF157 enhancer, SOX9 site scrambled, buffering site strengthened

Depositor

Joanna Wysocka

Insert

RNF157 (RNF157 Human)

Use

Luciferase

Mutation

RNF157 enhancer, SOX9 site scrambled, buffering s…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-PROTOP

Plasmid

#217091

Purpose

Donor plasmid of PROTOP Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of PROTOP transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP72

Plasmid

#196810

Purpose

Contains PMR4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

POWDERY MILDEW RESISTANT 4

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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