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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA

Plasmid

#162089

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-ProtC-HA

Use

Lentiviral

Tags

StrepTagII-ProtC-HA

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-FLAG

Plasmid

#162092

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-HA-FLAG

Use

Lentiviral

Tags

StrepTagII-HA-FLAG

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1

Plasmid

#162093

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-HA-AU1

Use

Lentiviral

Tags

StrepTagII-HA-AU1

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-FLAG

Plasmid

#162095

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-FLAG

Use

Lentiviral

Tags

ProtC-HA-FLAG

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

Plasmid

#162096

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-AU1

Use

Lentiviral

Tags

ProtC-HA-AU1

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

Plasmid

#162098

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA

Plasmid

#162080

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Publication

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-HA

Use

Lentiviral

Tags

VSVg-StrepTagII-HA

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern/EGFP

Plasmid

#179913

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Publication

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Publication

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-L207W-EFGP

Plasmid

#176900

Purpose

mouse ILK with L207W point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Publication

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Leucine 207 to Tryptophane

Promoter

CNV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Publication

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-PolB(T304I)-Puro

Plasmid

#177143

Purpose

Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PLOD2 sgRNA4

Plasmid

#136459

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Publication

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Publication

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Publication

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR10

Plasmid

#167003

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Publication

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 10 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-C-IDR

Plasmid

#166447

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-C-IDR(266-504) (YAP1, CRY2 Human)

Use

Lentiviral

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-TEAD4-IDR

Plasmid

#166449

Purpose

Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)

Use

Lentiviral

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

Plasmid

#162020

Purpose

Expression of GEF dead IQSEC1 mutant

Depositor

David Bryant

Publication

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Mutation

EK

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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