We narrowed to 165,978 results for: HTT

1. pFastBac HTB His-TEV-rDPP9-FLAG

   Plasmid

   #217328

   Purpose

   Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac HTB His-TEV-rDPP9(S729A)

   Plasmid

   #217327

   Purpose

   Purification of catalytically-dead rat DPP9 from insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   S729A

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-DDX53-FLAG

   Plasmid

   #222241

   Purpose

   Express FLAG tagged DDX53 in mammalian cells

   Depositor

   Yuliang Wu

   Insert

   DDX53 (DDX53 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29a-DDX53-Q249A

   Plasmid

   #222216

   Purpose

   Express DDX53 mutant Q249A protein in bacteria

   Depositor

   Yuliang Wu

   Insert

   DDX53_Q249A (DDX53 Human)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   K272A

   Promoter

   T7

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29a-DDX53-K272A

   Plasmid

   #222215

   Purpose

   Express DDX53 mutant K272A protein in bacteria

   Depositor

   Yuliang Wu

   Insert

   DDX53_K272A (DDX53 Human)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   K272A

   Promoter

   T7

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-DDX53-E377Q

   Plasmid

   #222217

   Purpose

   Express DDX53 mutant E377Q protein in bacteria

   Depositor

   Yuliang Wu

   Insert

   DDX53_E377Q (DDX53 Human)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   E377Q

   Promoter

   T7

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-DDX53-GFP

   Plasmid

   #222239

   Purpose

   Express GFP tagged DDX53 in mammalian cells

   Depositor

   Yuliang Wu

   Insert

   DDX53 (DDX53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-DDX53-K272A

   Plasmid

   #222242

   Purpose

   Express FLAG tagged DDX53 mutant K272A in mammalian cells

   Depositor

   Yuliang Wu

   Insert

   DDX53 (DDX53 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K272A

   Promoter

   T7

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L0I75_tUBQ10_GG5-GG6

   Plasmid

   #219714

   Purpose

   Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG5-tUBQ10-GG6
   

   Use

   Synthetic Biology

   Mutation

   WT terminator with added golden gate linkers

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC102-MBP-eIF4G-SkA

    Plasmid

    #219977

    Purpose

    eIF4G-SkA protein purification

    Depositor

    Simon Alberti

    Insert

    eIF4G-SkA (TIF4631 Budding Yeast)
    

    Tags

    6xHis-MBP

    Expression

    Insect

    Mutation

    eIF4A binding domain of S. kudriavzevii

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC102-MBP-eIF4G-RAR

    Plasmid

    #219973

    Purpose

    eIF4G-RAR protein purification

    Depositor

    Simon Alberti

    Insert

    eIF4G-RAR (TIF4631 Budding Yeast)
    

    Tags

    6xHis-MBP

    Expression

    Insect

    Mutation

    Truncation: amino acids 490 to 942

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFHMSPN-avi-TEV-LIC V

    Plasmid

    #220885

    Purpose

    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expresssion

    Tags

    Honeybee melittin signal followed by His, avi and…

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. L0I89-pMUTE_GG0-1

    Plasmid

    #219708

    Purpose

    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pMUTE-GG1
    

    Use

    Synthetic Biology

    Mutation

    WT promoter with added golden gate linkers

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-GST-MPK6

    Plasmid

    #215170

    Purpose

    Encodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thaliana

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    His-GST-MPK6
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 lac

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. L0I74-_tUBQ10_GG4-GG6

    Plasmid

    #219713

    Purpose

    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Mutation

    WT terminator with added golden gate linkers

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. L0I87-SPCHPromoter_GG0-1

    Plasmid

    #219707

    Purpose

    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH-GG1
    

    Use

    Synthetic Biology

    Mutation

    WT promoter with added golden gate linkers

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid

    #219715

    Purpose

    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-Bxb1(noBsaI0)-GG4
    

    Use

    Synthetic Biology

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mPLD3-sgRNA-Cas9-mcherry

    Plasmid

    #199700

    Purpose

    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MUT_S1-N

    Plasmid

    #218398

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MUT_N-H1

    Plasmid

    #218397

    Purpose

    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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