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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO2251

Plasmid

#141305

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI134

Plasmid

#126071

Purpose

Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/CURT_fluoB

Plasmid

#117997

Purpose

Expression of CURT_fluoB controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoB

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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P16ΔS:(MCS)-S11

Plasmid

#108184

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEM120

Plasmid

#250588

Purpose

H2Apr-SAS6-mClover3-SAS6ter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Apr-SAS6-mClover3-SAS6ter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pEM112C

Plasmid

#250587

Purpose

H2Apr-Lifeact-mClover3-H2Ater

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-Lifeact-mClover3-H2Ater

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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CAG-LifeCamp

Plasmid

#251723

Purpose

Expression of calcium lifetime sensor LifeCamp under CAG promoter

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-LifeCamp

Plasmid

#251724

Purpose

Expression of calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

LifeCamp

Use

AAV

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pEM110

Plasmid

#250586

Purpose

H2Apr-MRLC-mClover3-FLAG-MRLCter

Depositor

Lillian Fritz-Laylin

Article

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Bpr-P2A-T2A-HygR-Synther8:H2Apr-SpunMLRC-mClover3-SpunMLRCter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pNDGG015

Plasmid

#231336

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-p15A-Gm-ELT3; pNDGG021, pOGG009, pNDGG006, pOGG013. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG014

Plasmid

#231335

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG003

Plasmid

#231330

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H

Plasmid

#203901

Purpose

Reporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Insert

pD4H-FLUC-tOCS; pNOS-RLUC-tNOS

Use

Luciferase

Expression

Plant

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-po-mCerulean

Plasmid

#213861

Purpose

Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Peroxisome transit peptide fused mCerulean

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ221

Plasmid

#212319

Purpose

Express MmFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria guide

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB605-GBSSI

Plasmid

#213867

Purpose

Binary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Expression

Bacterial and Plant

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB560-GBSSI

Plasmid

#213866

Purpose

Binary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Expression

Bacterial and Plant

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03-Citrine-NLS

Plasmid

#188558

Purpose

Binary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

Citrine-NLS and MpIGPDm

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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