We narrowed to 11,193 results for: ENA
-
Plasmid#225314PurposeExpresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)
Plasmid#225306PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1
ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)
Plasmid#225307PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh2
ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)
Plasmid#233873PurposeExpresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eddh
TagsHisExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)
Plasmid#233876PurposeExpresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
TagsHis8ExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Kan
Plasmid#232102PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg
Plasmid#232101PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Nat
Plasmid#232094PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SOPP3-HA-nes
Plasmid#229071PurposeExpresses SOPP3 in the cytosol of mammalian cellsDepositorInsertExpress SOPP3 in the cytosol of mammalian cells
ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-K435L-peGFP-N1
Plasmid#228586PurposeExpresses OSCA1.2-K435L-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
ExpressionMammalianMutationK435LAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
TMEM63A-S475K-peGFP-N1
Plasmid#228592PurposeExpresses mouse TMEM63A-S475K-eGFP in mammalian cellsDepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM63A-A476K-peGFP-N1
Plasmid#228593PurposeExpresses mouse TMEM63A-A476K-eGFP in mammalian cellsDepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-A439K-peGFP-N1
Plasmid#228589PurposeExpresses OSCA1.2-A439K-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
ExpressionMammalianMutationA439KAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-Cut190*SS
Plasmid#218706PurposeEncodes codon optimized Cut190*SSenzyme for bacterial expressionDepositorInsertCut190*SS
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC-ICCG
Plasmid#218687PurposeEncodes codon optimized LCC-ICCG enzyme for bacterial expressionDepositorInsertLCC-ICCG
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-SfCut
Plasmid#218708PurposeEncodes codon optimized SfCut enzyme for bacterial expressionDepositorInsertSfCut
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
TagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK Promoter (MTK 2) (pAN2822)
Plasmid#194224PurposeMammalian Toolkit part 2 encoding the PGK promoterDepositorInsertPGK Promoter
UseSynthetic BiologyPromoterPGKAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a Promoter (MTK 2) (pAN2824)
Plasmid#194225PurposeMammalian Toolkit part 2 containing the EF1a promoterDepositorInsertEF1a Promoter
UseSynthetic BiologyPromoterEF1aAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-mTagBFP2
Plasmid#220747PurposemTagBFP2 expression in mammalian cells. Reporter plasmid.DepositorInsertmTagBFP2
ExpressionMammalianPromoter12xUAS with TATA-box minimal promoterAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph
Plasmid#194018PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
TagsHis10 and mEGFP,PromoterPolyhedrinAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_M15
Plasmid#220488Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_E23
Plasmid#220489Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speG_genomic
Plasmid#220473Purposeheterologous expression under endogenous speG E. coli promoterDepositorInsertspeG
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA4114
Plasmid#220474Purposeheterologous expression of PA4114 under endogenous speG E. coli promoterDepositorInsertPA4114
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1472
Plasmid#220475Purposeheterologous expression of PA1472 under endogenous speG E. coli promoterDepositorInsertPA1472
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1377
Plasmid#220476Purposeheterologous expression of PA1377 under endogenous speG E. coli promoterDepositorInsertPA1377
ExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN007
Plasmid#220048PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.DepositorInsertflag-HA-stop-GFP
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN013
Plasmid#220050PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.DepositorInsertGFP-stop-fLuc
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-mRFP
Plasmid#218709PurposeEncodes codon optimized mRFP enzyme for bacterial expressionDepositorInsertmRFP
Tags10xHisExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC-A2
Plasmid#218691PurposeEncodes codon optimized LCC-A2 enzyme for bacterial expressionDepositorInsertLCC-A2
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-IsPETase
Plasmid#218697PurposeEncodes codon optimized IsPETase enzyme for bacterial expressionDepositorInsertIsPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1
Plasmid#218704PurposeEncodes codon optimized PES-H1 enzyme for bacterial expressionDepositorInsertPES-H1
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL635
Plasmid#189829PurposeThe T-DNA backbone of Cas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsccdbPromoterZmUbi1Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL637
Plasmid#189832PurposeThe T-DNA backbone of vCas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna clonePromoterZmUbi1Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL639
Plasmid#189833PurposeThe T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna clonePromoterZmUbi1Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-P2A-Puro
Plasmid#211645PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF226_EFS-Cas9-P2A-Puro
Plasmid#211644PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_CbFDH
Plasmid#212140PurposeCargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.DepositorInsertformate dehydrogenase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0520 (pDEST, TRP1)
Plasmid#210481PurposepDEST vector with TRP1 marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseDestination vector for gateway cloningPromoterADH1Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only