We narrowed to 1,336 results for: cbs

1. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

   Plasmid

   #229810

   Purpose

   A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-AmCyan-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

   Plasmid

   #229813

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp-cFos
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

   Plasmid

   #229814

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

   Plasmid

   #229820

   Purpose

   An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

   Plasmid

   #229824

   Purpose

   A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

   Plasmid

   #229841

   Purpose

   A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-H2B-mCerulean-2xStop-loxP
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-Crestin-pro (JDW 1320)

   Plasmid

   #229830

   Purpose

   A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Crestin promoter, c-fos minimal promoter, and b-globin intron
   

   Use

   Gateway entry clone

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

   Plasmid

   #229838

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid

    #224474

    Purpose

    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid

    #224496

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

    Plasmid

    #224505

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexOP-35SCaMV
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

    Plasmid

    #224506

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-oMDC-LexOP-c-Fos
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid

    #224512

    Purpose

    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xHA-BirA-T2A-mCherry-Caax
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

    Plasmid

    #224522

    Purpose

    A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E 5X C120 c-fos min pro (JDW 1241)

    Plasmid

    #224544

    Purpose

    A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    5x-C120-c-Fos promoter
    

    Use

    Gateway cloning

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRHD-TetR-GAL80-Rho1-GAL4

    Plasmid

    #218274

    Purpose

    Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

    Depositor

    Christopher Howard

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
    

    Expression

    Yeast

    Mutation

    DHFR (P66L), Rho1 (C17R)

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRHDGAL43C

    Plasmid

    #218279

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRGAL43C

    Plasmid

    #218278

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRHDGAL3C

    Plasmid

    #218276

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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