We narrowed to 2,414 results for: organization Addgene

1. GFP-ERK2 K52R

   Plasmid

   #118298

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 K52R (Mapk1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-PTEF1-MCP-5M-ECM33_intron

   Plasmid

   #127600

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIS GEM-CEPIA1er

   Plasmid

   #58217

   Purpose

   Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   GEM-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GEM-GECO1 E298D F359W D400E E407D

   Promoter

   CAG

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-ERK2 del174-177

   Plasmid

   #118297

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 del174-177 (Mapk1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion 174aa-177aa

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mito-PAGFP

   Plasmid

   #23348

   Purpose

   Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

   Insert

   PAGFP
   

   Use

   Tags

   mito

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV R-CEPIA1er

   Plasmid

   #58216

   Purpose

   Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   R-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV G-CEPIA1er

   Plasmid

   #58215

   Purpose

   Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   G-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV CEPIA3mt

   Plasmid

   #58219

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA3mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV CEPIA4mt

   Plasmid

   #58220

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA4mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L F395W D436E

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PDZD7-TwinStrepII

    Plasmid

    #83198

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    PDZD7 (Pdzd7 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PITPbeta-GFP

    Plasmid

    #58274

    Purpose

    mammalian expression of GFP-PITP beta

    Depositor

    Shamshad Cockcroft

    Article

    Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. FHpHG_mTent5c_dCAT

    Plasmid

    #221442

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c_AA (dCAT) (Fam46c Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    D90A, D92A (GATctgGAT to GCCctgGCC)

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FHpHG_mTent5a

    Plasmid

    #221439

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5a (Fam46a Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FHpHG_mTent5c

    Plasmid

    #221440

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c (Fam46c Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Htr5b(3.7)-tTA-sv40

    Plasmid

    #184412

    Purpose

    AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-tTA
    

    Use

    AAV

    Tags

    tTA

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. ANKRD24-TwinStrepII

    Plasmid

    #83190

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    ANKRD24 (Ankrd24 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge