We narrowed to 5,650 results for: pCas

1. SpCas9-NRCH-SsAPOBEC3B

   Plasmid

   #198550

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-SsAPOBEC3B
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Promoter

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Promoter

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #86613

   Purpose

   Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-ChroME-mRuby2-ST

   Plasmid

   #108902

   Purpose

   Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-CoChR-mRuby2-ST

   Plasmid

   #109131

   Purpose

   Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   CoChR-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCambia2300 - ProRUBY:RUBY-Citrine

    Plasmid

    #128796

    Purpose

    Fluorescent reporter for subcellular localisation and expression analysis of RUBY

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

    Insert

    RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    ProRUBY

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 35S::GeNL/pCAMBIA1301

    Plasmid

    #190070

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG mEmerald-EB3

    Plasmid

    #168514

    Purpose

    Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Mutation

    Promoter

    osk

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMUT2-pCadBA-mCherry

    Plasmid

    #192865

    Purpose

    pMUT2 based vector for sensing pH in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    pH sensitive promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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