Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,616 results for: pCas

Clear keywords
Showing: 1081 - 1100 of 2616 results

  9. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

    Enlarge

  10. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

    Enlarge

  11. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

    Enlarge

  14. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid
    #223112
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223112-plasmid-map-sequence-id-447635

    Enlarge

  15. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid
    #223115
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223115-plasmid-map-sequence-id-447640

    Enlarge

  16. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid
    #223116
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223116-plasmid-map-sequence-id-447639

    Enlarge

  17. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

    Enlarge

  19. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid
    #197505
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…
    Promoter
    CMV and T7
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197505-plasmid-map-sequence-id-386783

    Enlarge

  20. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

    Enlarge

Showing: 1081 - 1100 of 2616 results