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Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
UseTagsExpressionBacterialMutationSynonymous point mutation in the wobble positionā¦PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-V5His6_R
Plasmid#147400PurposeInsect Expression of CeAIN2_254-400DepositorInsertCeAIN2_254-400
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compareā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W282AW390A_O
Plasmid#147143PurposeInsect Expression of CeAIN2-W282AW390ADepositorInsertCeAIN2-W282AW390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W366A_O
Plasmid#147144PurposeInsect Expression of CeAIN2-W366ADepositorInsertCeAIN2-W366A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W366AW390A_O
Plasmid#147145PurposeInsect Expression of CeAIN2-W366AW390ADepositorInsertCeAIN2-W366AW390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W390A_O
Plasmid#147146PurposeInsect Expression of CeAIN2-W390ADepositorInsertCeAIN2-W390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-3xWmut_O
Plasmid#147139PurposeInsect Expression of CeAIN2-3xWmutDepositorInsertCeAIN2-3xWmut
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W282A_O
Plasmid#147141PurposeInsect Expression of CeAIN2-W282ADepositorInsertCeAIN2-W282A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W282AW366A_O
Plasmid#147142PurposeInsect Expression of CeAIN2-W282AW366ADepositorInsertCeAIN2-W282AW366A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared ā¦PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R
Plasmid#147407PurposeInsect Expression of CeAIN2_254-400DepositorInsertCeAIN2_254-400
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compareā¦PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP*
Plasmid#175237PurposeBacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5233
Plasmid#128715PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcM-hopM1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4
Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCPP5951
Plasmid#128719PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopR1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4
Plasmid#62900PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-Gal4
Plasmid#62901PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3flag-RanGAPdeltaN
Plasmid#25104DepositorInsertRanGAP1 deleted N-term domain (RANGAP1 Human)
UseTagsFlagExpressionMammalianMutationDeleted amino acids 1-203.PromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only