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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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L1-Ck2-smR-chlo

Plasmid

#163939

Purpose

L1 in position 2, for spectinomycin in chloroplast transformation

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

aadA gene for spectinomycin resistance

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Ntpsba_rev_chlo

Plasmid

#163932

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Nicotiana tabacum psba terminator

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_eGFP

Plasmid

#136095

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_mTurquoise2

Plasmid

#136104

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mTurquoise2(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mVenus

Plasmid

#136106

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mVenus(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS_nptII

Plasmid

#136080

Purpose

Confers plant resistance to kanamycin

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_nptII

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS_hptII

Plasmid

#136082

Purpose

Confers plant resistance to hygromycin

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_hptII

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_eGFP

Plasmid

#136085

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_eGFP(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_mVenus

Plasmid

#136091

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mVenus(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3919-32

Plasmid

#176496

Purpose

pCAG-puro-T2A-TetON3G-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

puroR-T2A-TetOn3G

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EC0_3_Mxi1

Plasmid

#163715

Purpose

Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

Mxi1+SV40 NLS

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC0_6_sgRNA

Plasmid

#163717

Purpose

Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

sgRNA expression cassette

Use

Synthetic Biology; Moclo vector level 0 (type 234)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC0_4_VPR

Plasmid

#163716

Purpose

Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

VPR

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3

Plasmid

#165779

Purpose

3xP3 eye driver enhancer element, requires promoter element for function

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3xP3

Expression

Insect

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O1

Plasmid

#165843

Purpose

_C31 integrase attB site in pColE1_Omega1

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFC31 attB O2

Plasmid

#165844

Purpose

_C31 integrase attB site in pColE1_Omega2

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 attB

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-O2

Plasmid

#165816

Purpose

Omega2 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-O2

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p4xLexAOp-O1

Plasmid

#165817

Purpose

Omega1 4xLexAOp for assembly of larger LexAOp response elements

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

4xLexAOp-O1

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFBD

Plasmid

#165823

Purpose

QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFBD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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