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CAG-NLS-mScarleti-NLS-IRES-Puromycin

Plasmid

#234890

Purpose

Expressed a nuclear mScarleti fluorophoro in mammalian cells

Depositor

Nancy Papalopulu

Article

Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

Insert

mScarleti

Tags

NLS on N- and C- terminals

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSE89: pFuGW-G5p-EYFP

Plasmid

#240461

Purpose

Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

G5p
EYFP

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE92: pFuGW-G14p-EYFP

Plasmid

#240462

Purpose

A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites (G14p) regulating EYFP

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

EYFP
G14p

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

Plasmid

#240455

Purpose

SARS-CoV-2 Protease 3CLpro expression plasmid

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Insert

3CLpro

Use

Lentiviral

Promoter

hUbCp

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Merlin(dog)gRNA1

Plasmid

#214823

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-Merlin(dog)gRNA2

Plasmid

#214824

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-chGrb2/eDHFR(69K6)-iRFP713

Plasmid

#214828

Purpose

Encoding Grb2-eDHFR(69K6) chimera fused to iRFP713

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713

Tags

iRFP713

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-GG

Plasmid

#213039

Purpose

AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)

Depositor

Fredrik Sterky

Article

De La Cruz et al Bio Protoc. 2024 Nov 5;14(21):e5097. doi

Type

Empty backbone

Use

AAV; Vector for golden gate assembly

Tags

N/A

Promoter

N/A

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-iS-methadoneSnFR-Golgi

Plasmid

#220865

Purpose

Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12-Clover

Plasmid

#215527

Purpose

Expression vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12

Plasmid

#215517

Purpose

Dox-inducible lentiviral transfer vector with PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12-Clover

Plasmid

#215521

Purpose

Gateway entry vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(E378G)

Plasmid

#215462

Purpose

Gateway entry vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

E378G (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-VPS35-mScarlet

Plasmid

#215472

Purpose

Gateway entry vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Use

Gateway entry vector

Tags

mScarlet

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(Y527F)

Plasmid

#215460

Purpose

Gateway entry vector with Src constitutively-active mutant (Y527F)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

Y527F (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(K295R)

Plasmid

#215461

Purpose

Gateway entry vector with Src kinase-dead mutant (K295R)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

K295R (Kinase dead)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE2-EF1alpha TDH

Plasmid

#236457

Purpose

stable expression of TDH using viral infection

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

TDH (Tdh Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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