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pLAS2w.Phyg-CHST8

Plasmid

#232650

Purpose

expresses CHST8 wild-type in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAM88

Plasmid

#226680

Purpose

Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

rpt1 (RPT1 Budding Yeast)
Rpt2 (RPT2 Budding Yeast)
Rpt3 (RPT3 Budding Yeast)
Rpt4 (RPT4 Budding Yeast)
Rpt5 (RPT5 Budding Yeast)
Rpt6 (RPT6 Budding Yeast)

Tags

FLAG and His

Expression

Bacterial

Mutation

I191TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM89

Plasmid

#226681

Purpose

Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

rpt1 (RPT1 Budding Yeast)
Rpt2 (RPT2 Budding Yeast)
Rpt3 (RPT3 Budding Yeast)
Rpt4 (RPT4 Budding Yeast)
Rpt5 (RPT5 Budding Yeast)
Rpt6 (RPT6 Budding Yeast)

Tags

FLAG and His

Expression

Bacterial

Mutation

Q49TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

Plasmid

#215910

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332D, S1346D, S1616D, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215911

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-NSD3

Plasmid

#221297

Purpose

Expression of NSD3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NSD3 (pLxIS) (Nsd3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_V47N_I48N

Plasmid

#214319

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

V47N_I48N

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_LES33AAA

Plasmid

#214321

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

LES33AAA

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_DDEN29ENGD

Plasmid

#214320

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

DDEN29ENGD

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_E192K_D193K

Plasmid

#214318

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

E192K_D193K

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Tags

FLAG and His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-ZNF746

Plasmid

#207822

Purpose

Dual expression of Cas9 and sgRNA targeting ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 sgRNA (ZNF746 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA

Plasmid

#206179

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S192A, S194A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS

Plasmid

#206180

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA

Plasmid

#206181

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S194A

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S13S2DBD

Plasmid

#200196

Purpose

Expression of Sox13 with Sox2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S13S2DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)

Promoter

EF_1alpha

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S2S13DBD

Plasmid

#200195

Purpose

Expression of Sox2 with Sox13 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S2S13DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox2(1-126); Sox13 (1242-1449); Sox2 (333-957)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)

Plasmid

#199570

Purpose

Stable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 isoform E (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Mutation

codon optimized

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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