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pX459-Arf1 gRNA

Plasmid

#246572

Purpose

CRISPR vector co-expressing Cas9 and a mouse Arf1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Arf1 gRNA (Arf1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd4 gRNA

Plasmid

#246563

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd4 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd4 gRNA (Fzd4 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itga5 gRNA

Plasmid

#246573

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itga5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itga5 gRNA (Itga5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd5 gRNA

Plasmid

#246564

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd5 gRNA (Fzd5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd8 gRNA

Plasmid

#246565

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd8 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd8 gRNA (Fzd8 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd3 gRNA

Plasmid

#246552

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd3 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd3 gRNA (Fzd3 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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SSH-3 WT

Plasmid

#245957

Purpose

Expression of wild-type slingshot 3, a cofilin pSer3 phosphatase

Depositor

James Bamburg, Kensaku Mizuno, Kazumasa Ohashi

Article

Ohta et al Genes Cells. 2003 Oct;8(10):811-24. doi:

Insert

Slingshot protein phosphatase 3 (Ssh3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1261

Plasmid

#235726

Purpose

Protein expression of His6-SUMO-human RAB5A Q79L 1-211 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5A (RAB5A Human)

Tags

6xHis-SUMO-TEV

Expression

Bacterial

Mutation

Q79L, aa 1-211

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Cd109 gRNA

Plasmid

#246571

Purpose

CRISPR vector co-expressing Cas9 and a mouse Cd109 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Cd109 gRNA (Cd109 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd9 gRNA

Plasmid

#246551

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd9 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd9 gRNA (Fzd9 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itgav gRNA

Plasmid

#246576

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itgav gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itgav gRNA (Itgav Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β1β6-EGFP

Plasmid

#242826

Purpose

Encodes human integrin β1 chimera containing integrin β6 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 1, transcript variant 1A (ITGB1 Human)

Expression

Mammalian

Mutation

Integrin β1 chimera containing the cytoplasmic ta…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔSEMA

Plasmid

#190647

Purpose

Expresses Mouse Sema7A with SEMA domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

SEMA Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔIG

Plasmid

#190648

Purpose

Expresses Mouse Sema7A with IG Domain Deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

IG Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔPSI

Plasmid

#190649

Purpose

Expresses Mouse Sema7A with PSI domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

PSI Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-KCE

Plasmid

#190651

Purpose

Expresses Mouse Sema7A with RGD motif mutated to KCE

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

RGD mutated to KCE

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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