We narrowed to 63,974 results for: cell

1. pLVX-Puro-TDP-43-K263E

   Plasmid

   #133758

   Purpose

   Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed lysine 263 to glutamate.

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CLYBL-TO-PAX7

   Plasmid

   #204346

   Purpose

   Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   human PAX7
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST40-2XHA-Src-Y530F

   Plasmid

   #140318

   Purpose

   Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-β-tubulin-mCherry

   Plasmid

   #175829

   Purpose

   Express mCherry with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with microtuble-targeting sequence
   

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

   Plasmid

   #118375

   Purpose

   Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-BirA*

   Expression

   Mammalian

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-C1-mTrim21ΔRING-Box

   Plasmid

   #105517

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   CMV

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-C1-mTrim21ΔPRYSPRY

   Plasmid

   #105518

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   CMV

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-DEPTOR-WT

   Plasmid

   #159159

   Purpose

   Expresses DEPTOR in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDARMO_CMVT_FLAG-HUWE1

   Plasmid

   #187121

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_PGK_LaG17-mCherry-HOTag3

    Plasmid

    #190024

    Purpose

    Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    LaG17 CluMPS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-GGA1

    Plasmid

    #178459

    Purpose

    Expresses GGA1 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

    Insert

    GGA1 (GGA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCit(P5f-P13f)3-gs

    Plasmid

    #228080

    Purpose

    Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, P5f, P13f
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TagBFP(P6f)3(P14f)3-gs

    Plasmid

    #228081

    Purpose

    Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, P6f, P14f
    

    Expression

    Mammalian

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5'-BirA-FLAG GFP minimal CMV pCDNA5

    Plasmid

    #212087

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAuroraA-GFP-AURKA_K162M-mCherry

    Plasmid

    #99880

    Purpose

    Expression of GFP-AURKA K162M-mCherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    K162M (kinase-dead)

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LZRS-hNEDD9_IresGFP

    Plasmid

    #21962

    Depositor

    Lynda Chin

    Article

    Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

    Insert

    NEDD9 (NEDD9 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pDEST_FLAG-HUWE1_C4341S

    Plasmid

    #187156

    Purpose

    mammalian cell expression FLAG-HUWE C4341S

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    C4341S

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lyn11-BirA*-HA

    Plasmid

    #214474

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and Lyn11 (Plasma Membrane Localization Se…

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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