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pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C

Plasmid

#146015

Purpose

Insect Expression of DmSmaug-del584-762

Depositor

Elisa Izaurralde

Insert

DmSmaug-del584-762 (smg Fly)

Expression

Insect

Mutation

7 silent mutations and a deletion of the AA 961-9…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

Plasmid

#147007

Purpose

Insect Expression of DmUPF3-K31E-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF3-K31E-siRNAres (Upf3 Fly)

Expression

Insect

Mutation

5 silent and one non silent mutation P305S compar…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

Plasmid

#146990

Purpose

Insect Expression of DmUPF1_92-1180-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF1_92-1180-siRNAres (Upf1 Fly)

Expression

Insect

Mutation

1 non silent mutations: G995E compared to the seq…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCasTet-λ

Plasmid

#128318

Purpose

Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

Depositor

Christopher Johnston

Article

Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

Insert

TetR and pTetR/TetO

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Constitutive expression of cas9 and anhydro-tetra…

Promoter

tet promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z

Plasmid

#148157

Purpose

Insect Expression of Dm4E-T_1-511-del28-43

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-del28-43 (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

Plasmid

#148158

Purpose

Insect Expression of Dm4E-T_1-511-FWKtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-FWKtoAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z

Plasmid

#148161

Purpose

Insect Expression of Dm4E-T_1-511-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-Y10AL15A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z

Plasmid

#148162

Purpose

Insect Expression of Dm4E-T_1-511-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-YLLLtoAAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y

Plasmid

#148070

Purpose

Insect Expression of DmPAN2-del1047-1063-GSG-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del1047-1063-GSG-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared the the sequence gei…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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