We narrowed to 1,254 results for: sumo

1. pTXG-hTDG-K330A

   Plasmid

   #52282

   Purpose

   Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   hTDG (TDG Human)
   

   Use

   Tags

   GyrA-intein-GST

   Expression

   Bacterial

   Mutation

   K330A, SUMO acceptor site mutation

   Promoter

   T7

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXG-hXRCC1-K176R

   Plasmid

   #52284

   Purpose

   Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Tags

   GyrA-intein-GST

   Expression

   Bacterial

   Mutation

   K176R, SUMO acceptor site mutant

   Promoter

   T7

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. MZB139

   Plasmid

   #217838

   Purpose

   Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   10x His and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208040

   Purpose

   Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, TurboID

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSA2

   Plasmid

   #52262

   Purpose

   Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-2 (SUMO2 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-PIAL2M-sim1

   Plasmid

   #67908

   Purpose

   Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496; VEDL 425-428 AAA

   Promoter

   P-lac

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-SENP7 (catalytic domain)

   Plasmid

   #16360

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP7 catalytic domain (SENP7 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 640 to 984 catalytic domain of human S…

   Promoter

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

8. RanBP2-RB3-4-pET23a

   Plasmid

   #85769

   Purpose

   bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanBP2 fragment (RANBP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   fragment (amino acids 2304-3062)

   Promoter

   T7

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only