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Showing: 2751 - 2800 of 4578 results

  1. pNK2657

    Plasmid
    #219741
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    hispidin- synthase from Mycena citricolor
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219741-plasmid-map-sequence-id-437095

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  2. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Article
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Use
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  3. pNK115

    Plasmid
    #219745
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219745-plasmid-map-sequence-id-450697

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  4. pNK116

    Plasmid
    #219742
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    D37E, V181I, S323M, M385K
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219742-plasmid-map-sequence-id-437105

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  7. FLINC-AKAR1-CAAX

    Plasmid
    #87705
    Purpose
    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1-CAAX
    Use
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87705-plasmid-map-sequence-id-176711

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  13. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid
    #58422
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids P29 to D408
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58422-plasmid-map-sequence-id-90253

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  31. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  33. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  41. Tom20-mChF-AIP(TE)

    Plasmid
    #61522
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TE mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

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  49. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225950-plasmid-map-sequence-id-446852

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  50. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Richard Eva, Bart Nieuwenhuis
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225938-plasmid-map-sequence-id-446791

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Showing: 2751 - 2800 of 4578 results