We narrowed to 24,474 results for: Spr;

1. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

   Plasmid

   #176535

   Purpose

   Non-protein coding locus components from CRISPR-associated IscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

   Plasmid

   #216871

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

   Insert

   Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
   

   Use

   CRISPR

   Mutation

   mdx mutation in mouse dystrophin

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

   Plasmid

   #185489

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #228555

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   NLS-HA and P2A-mCherry

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228557

    Purpose

    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dRfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228558

    Purpose

    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dhfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)

    Plasmid

    #238168

    Purpose

    Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    CD55 sgRNA CRISPRa (CD55 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid

    #238169

    Purpose

    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    CD45 sgRNA CRISPRa (PTPRC Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

    Plasmid

    #192161

    Purpose

    AAV-CRISPRa base vector

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Type

    Empty backbone

    Use

    AAV

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone)

    Plasmid

    #238188

    Purpose

    Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    EGR3 sgRNA CRISPRa (EGR3 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668

    Plasmid

    #238174

    Purpose

    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 sgRNA CRISPRa (KLF4 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)

    Plasmid

    #238189

    Purpose

    Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    PLAGL1 sgRNA CRISPRa (PLAGL1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666

    Plasmid

    #238172

    Purpose

    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665

    Plasmid

    #238173

    Purpose

    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

    Plasmid

    #238177

    Purpose

    Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    HES7 sgRNA CRISPRa (HES7 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge