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pGBT9-GGA1-GAE

Plasmid

#199174

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA1-GAE domain (GGA1 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGST-parallell2-GGA3(571-723)

Plasmid

#199150

Purpose

Expresses GST-GGA3(571-723) in bacteria

Depositor

Juan Bonifacino

Article

Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

Insert

GGA3(571-723) (GGA3 Human)

Tags

GST and TEV protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-Rabex-5

Plasmid

#197043

Purpose

Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-lox

Plasmid

#190059

Purpose

Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lox

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-codA

Plasmid

#190058

Purpose

Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

codA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pYjjZ-RBS- sfGFP

Plasmid

#190054

Purpose

GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pKatG-RBS- sfGFP

Plasmid

#190053

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pOxyS-RBS- sfGFP

Plasmid

#190052

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-2

Plasmid

#180392

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-2 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-3

Plasmid

#180393

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-3 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCBH-Cas9-Suntag-BB_BbsI

Plasmid

#164805

Purpose

REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

Plasmid

#133795

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

Plasmid

#133797

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T16A)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T16A

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

Plasmid

#133799

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Leu-AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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